BD LSR II | Flow Cytometer | For Sale

Preowned Becton Dickinson LSR II For Sale

Year:  2007 (November)
S/N# : H47200095

Includes:
• Computer Workstation
• Fluidics Cart
• HTS Option
• Computer Workstation with FACSDiva 6.1
• Operator's Guide
• Communication & Power Cables
(Images Show Two Color System with Five Color Detection.  Site Inspections Are Welcome)

Product Description:

The BD LSR II flow cytometer is fully configurable with one to four fixed-aligned, air-cooled lasers. By distributing a large number of fluorochromes over four lasers, you reduce spectral overlap and improve sensitivity. Fixed alignment provides consistent and stable day-to-day performance. The three new solid state lasers available on the BD LSR II flow cytometer—the Coherent Sapphire, Coherent VioFlame PLUS, and Lightwave Xcyte— consume less power, produce less heat, and have a  lower total cost over the life of the laser compared  to gas-filled lasers.

The solid state Coherent Sapphire blue laser (20 mW @ 488 nm) produces no plasma glow, therefore signal-to-noise ratios are improved in 488 nm–excited fluorochromes.

The Coherent VioFlame PLUS violet laser (25 mW @ 405 nm) excites Cascade Blue, Pacific Blue, and Alexa Fluor 405 dyes, once used only with expensive Krypton lasers. In addition, four-color fluorescent protein experiments are easily performed using BD Clontech Living Colors
fluorochromes GFP, YFP, dsRed, and CFP with this laser.

The BD LSR II is the first commercially available flow cytometer to use a solid state UV laser. No special power or cooling requirements are needed, eliminating expensive room renovation costs. The Lightwave Xcyte laser (20 mW @ 355 nm) has outstanding performance on UV dyes such as DAPI, Hoechst, and Indo-1. UV DNA dyes, calcium flux, and stem cell side population experiments used to be run only on complex cell sorters. Now, with the benchtop BD LSR II flow cytometer, they are routine experiments.

With the addition of the HeNe laser (18 mW @ 633 nm), APC and APC-Cy7 dyes can be detected. The optical system also allows the simultaneous detection of Alexa Fluor 700 conjugates with APC and APC-Cy7. Multiple laser, detector, and fluorochrome options make the BD LSR II flow cytometer system the most versatile benchtop analyzer on the market today.

For More Information, Please Contact Joe LaFrange at JLaFrange@ssllc.com